Leafy Stems of Phagnalon saxatile subsp. saxatile from Algeriaas a Source of Chlorogenic Acids and Flavonoids with Antioxidant Activity: Characterization and Quantification Using UPLC-DAD-ESI-MSn

Phagnalon saxatile subsp. is a wild species widespread in Algeria which utilized for medicinal purposes as analgesic and anticholesterolemic. However, information still scarce regarding its phytochemical content. The objective of this study was to identify quantify the phenolic compounds from different extracts leafy stems. For purpose, effects four extracting solvents were investigated on content antioxidant activity plant. prepared with polar (methanol water) contained higher amounts showed better than apolar (hexane, dichloromethane). methanolic extract, richest total flavonoid, had significant regarded by DPPH° scavenging capacity (IC50 5.5 µg/mL), ABTS+° 63.8 µg/mL) inhibition oxidation linoleic acid 22.7 when compared synthetic antioxidants. Chlorogenic acids several flavonoids identified quantified UPLC-DAD-MSn. di-O-caffeoylquinic isomers most concentrated phenolics (25.4 mg/g DW) extract.